Cardiac Biomarker ELISA's
Life Diagnostics, Inc. manufactures ELISA kits for measurement of cardiac troponin-I, myoglobin, fatty acid binding protein (FABP) and myosin light chain-1 (MLC-1) in species including mouse, rat, guinea pig, rabbit, dog pig and monkey. A list of our cardiac
biomarker ELISA's is shown below. Kit details and instructions can be obtained by clicking on the "insert" buttons. Orders may be placed online or by phone, fax or email.
IMPORTANT: Serum or plasma that is to be used for measurement of cardiac biomarkers must not be collected by cardiac puncture or any other method that causes damage to the heart. Cardiac puncture causes release of very high levels of cardiac biomarkers into the blood thereby invalidating study results.
Literature references detailing the use of our cardiac biomarker ELISA kits are listed at the bottom of this page.
Cardiac Troponin-I ELISA Kits |
Kit Name |
Kit Insert |
Catalog # |
Quantity |
Unit Price |
|
|
Mouse Cardiac Tn-I 96-well ELISA
(For use with serum, 0.156 - 10 ng/ml) |
insert |
2010-1-HS |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
|
Mouse Cardiac Tn-I 96-well ELISA
(For use with plasma, 0.156 - 10 ng/ml)
Please note that as of 5/19/10 the range of thekit has been changed from 0.078 - 5 ng/ml to 0.156 - 10 ng/ml |
insert |
2010-1-HSP |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
|
Rat Cardiac Tn-I 96-well ELISA
(For use with serum, 0.156 - 10 ng/ml) |
insert |
2010-2-HS |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
Rat
Cardiac Tn-I 96-well ELISA
(For use with plasma, 0.156 - 10 ng/ml)
Please note that as of 5/19/10 the range of thekit has been changed from 0.078 - 5 ng/ml to 0.156 - 10 ng/ml |
insert |
2010-2-HSP |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
|
Dog Cardiac Tn-I 96-well ELISA
(For use with serum, 0.156 - 10 ng/ml) |
insert |
2010-3-HS |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
Pig
Cardiac Tn-I 96-well ELISA
(For use with serum, 0.039 - 2.5 ng/ml)
|
insert |
2010-4-HS |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
|
Pig Cardiac Tn-I 96-well ELISA
(For use with plasma, 0.039 - 2.5 ng/ml) |
insert |
2010-4-HSP |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
Monkey Cardiac Tn-I 96-well ELISA
(For use with serum, 0.156 - 10 ng/ml) |
insert |
2010-5-HS |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
Rabbit Cardiac Tn-I 96-well ELISA
(For use with serum, 0.156 -10 ng/ml) |
insert |
2010-6-HS |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
Guinea Pig Cardiac Tn-I 96-well ELISA
(For use with serum, 0.2 -12.5 ng/ml) |
insert |
2010-7-HS |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
|
Myoglobin ELISA Kits |
Kit Name |
Kit Insert |
Catalog # |
Quantity |
Unit Price |
|
|
Mouse Myoglobin 96-well ELISA
(New higher sensitivity version, for serum, plasma & urine samples
(3.9 - 250 ng/ml)
|
insert |
2110-1-N |
1 x 96-well |
$425 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
Rat
Myoglobin 96-well ELISA
(New higher sensitivity version, for
serum, plasma & urine samples,
1.56 - 100 ng/ml) |
insert |
2110-2-N |
1 x 96-well |
$425 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
|
Rabbit Myoglobin 96-well ELISA
(New higher sensitivity version, for serum, plasma & urine samples,
2 - 125 ng/ml)
|
insert |
2110-3-N |
1 x 96-well |
$425 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
Dog
Myoglobin 96-well ELISA
(New higher sensitivity version, for serum, plasma & urine samples,
3.9 - 250 ng/ml) |
insert |
2110-4-N |
1 x 96-well |
$425 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
|
Pig Myoglobin 96-well ELISA
(New higher sensitivity version, for serum, plasma & urine samples,
0.5 - 31.25 ng/ml)
|
insert |
2110-5-N |
1 x 96-well |
$425 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
|
Monkey Myoglobin 96-well ELISA
(New higher sensitivity version, for serum, plasma & urine samples,
2 - 125 ng/ml)
|
insert |
2110-6-N |
1 x 96-well |
$425 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
Guinea Pig Myoglobin 96-well ELISA
(High sensitivity version, for serum, plasma & urine samples,
1.56 - 100 ng/ml)
|
insert |
2110-7-N |
1 x 96-well |
$425 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
|
Fatty Acid Binding Protein ELISA Kits |
Kit Name |
Kit Insert |
Catalog # |
Quantity |
Unit Price |
|
|
Mouse Cardiac FABP 96-well ELISA
(0.31 - 20 ng/ml) |
insert |
2310-1-HS |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
Rat
Cardiac FABP 96-well ELISA
(0.078 - 5 ng/ml) |
insert |
2310-2-HS |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
|
Dog Cardiac FABP 96-well ELISA
(0.31 - 20 ng/ml) |
insert |
2310-4 |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
|
Pig Cardiac FABP 96-well ELISA
(0.78 - 25 ng/ml) |
insert |
2310-5 |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
|
Rabbit Cardiac FABP 96-well ELISA
(1.56 - 100 ng/ml) |
insert |
2310-6 |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
Cardiac Myosin Light Chain-1 ELISA |
Kit Name |
Kit Insert |
Catalog # |
Quantity |
Unit Price |
|
Rat & Mouse Cardiac MLC-1 96-well ELISA
(0.078 - 2.5 ng/ml) |
insert |
2320-2 |
1 x 96-well |
$450 |
|
2 x 96 well |
$800 |
|
4 x 96-well |
$1500 |
|
| |
The figure below illustrates the utility of our animal troponin-I, myoglobin and FABP kits. It shows the levels of the
respective markers in plasma obtained from a rabbit cardiac ischemia/reperfusion model. Plasma samples were collected before/during ischemia and at various times
after reperfusion. Consistent with clinical findings in humans, it can be seen that the (i) levels of myoglobin and FABP increase prior to those of troponin-I
and (ii) troponin-I levels remain elevated longer than do those of myoglobin and FABP.
Literature Citations
Mouse cTnI Publications
-
Richer MJ, Lavallée DJ, Shanina I, Horwitz MS (2009) Toll-Like Receptor 3 Signaling on Macrophages Is Required for Survival Following Coxsackievirus B4 Infection. PLoS ONE 4(1): e4127. doi:10.1371/journal.pone.0004127
-
Buyse MG, et.al. Long-term blinded placebo-controlled study of SNT-MC17/idebenone in the dystrophin deficient mdx mouse: cardiac protection and improved exercise performance. Eurpoean Heart Journal 30:116-124 (2009)
-
Elrod JW et.al. Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function. PNAS 104:15560-15565 (2007)
-
Eckle T, et.al. Cardioprotection by Ecto-5'-Nucleotidase (CD73) and A2B Adenosine Receptors. Circulation 115:1581-1590 (2007)
-
Eckle T, et.al. Systematic evaluation of a novel model for cardiac ischemic preconditioning in mice. Am J Physiol Heart Circ. Physiol. 291:H2533-H2540 (2006)
-
Hardarson HS, et.al. Toll-like receptor 3 is an essential component of the innate stress response in virus-induced cardiac injury. Am J Physiol Heart Circ. Physiol 292:H251-H258 (2006)
-
Madonna R, Rinaldi L, Rossi C, Geng Y-J and De Caterina R. Prostacyclin improves transcoronary myocardial delivery of adipose tissue-derived stromal cells. Clinical Research 27:2054-2061 (2006)
-
Morrison RR, Tan XL, Ledent C, Mustafa SJ and Hifmann PA. Targeted deletion of A2A adenosine receptors attenuates the protective effects of myocardial postconditioning. Am J Physiol Heart Circ Physiol. 293:H2523-H2529 (2007)
-
Engle, SK et al. Strain differences in serum cardiac troponin-I concentration and myocardial necrosis after isoproterenol treatment in mice. Abstract No. 383. The Toxicologist CD — An official Journal of the Society of Toxicology, Volume 96, Number S-1, March 2007.
-
Hou G, Dick R, Zeng C, Brewer GJ., Comparison of lowering copper levels with tetrathiomolybdate and zinc on mouse tumor and doxorubicin models. Translational Research 148:309-314 (2006)
-
Huber SA, Feldman AM and Sartini D., Coxsackievirus B3 induces T regulatory cells, which inhibit cardiomyopathy in tumor necrosis factor-a transgenic mice. Circ Res. 99:1109-1116 (2006)
-
Mullick A, Leon Z,
Min-Oo G, Berghout J, Lo R, Daniels E and Gros P. Cardiac failure in
C5-deficient mice after Candida albicans infection. Infection and Immunity.
74(8):4439-4451 (2006)
Hou G, Dick R, Abrams GD, Brewer GJ., Tetrathiomolybdate protects against cardiac damage by doxorubicin in mice. J Lab Clin Med.146(5):299-303 (2005
Tao L, Liu H-R, Gao F, Qu Y, Christopher TA, Lopez BL and Ma XL. Mechanical traumatic injury
without circulatory shock causes cardiomyocyte apoptosis: rol of reactive nitrogen and reactive oxygen species. Am J Heart Circ Physiol.
288:H2811-H2818 (2005)
Rat cTnI Publications
-
Yue T-L, et.al. In vivo activation of peroxisome proliferator-activated redeptor-d protects the heart from ischemia/reperfusion injury in Zucker fatty rats. J. Pharm. Exp. Ther. 325:466-474 (2008)
-
Zagorski J, Gellar MA, Obraztsova M, Kline JA and Watts JA. Inhibition of CINC-1 decreases right ventricular damage caused by experimental pulmonary embolism in rats. The Journal of Immunology 179:7820-7826 (2007)
-
Legare JF, Oxner A, Heimrath O, Myers T and Currie RW. Heat Shock treatment results in increased recruitment of labeled PMN following myocardial infarction. Am J Physiol Heart Circ Physiol. 293(5):H3210-5 (2007)
-
Chopra M and Sharma AC. Distinct cardiodynamic and molecular chracteristics during early and late stages of sepsis-induced myocardial dysfunction. Life Sciences. epub. July 2007
-
Liu J, Pang Y, Chang T, Bounelis P, Chatham JC, and Marchase RB. Increased hexoseamine biosynthesis
and protein O-GlcNAc levels associated with myocardial protection against calcium paradox and ischemia. J Mol. Cell Cardiol. 40 303-312 (2006)
-
Wang P and Chatham JC. Onset of diabetes in Zucker diabetic fatty (ZDF) rats leads to improved recovery of
function after ischemia in the isolated perfused heart. Am J Physiol Endocrinol Metab 286:E725-E736 (2004)
Rabbit cTnI Publications
-
Booth EA, and Lucchesi BR. Medroxyprogesterone acetate prevents the cardioprotective and anti-inflammatory effects of 17beta-estradiol in an in vivo model of myocardial ischemia and reperfusion. Am J Physiol Heart Circ Physiol. 293(3):H1408-15 (2007)
-
Lauver DA, Booth EA, White AJ, Poradosu E & Lucchesi BR. Sulodexide attenuates myocardial
ischemia/reperfusion injury and the deposition of C-reactive protein in areas of infarction without affecting hemostasis. J Pharm. Exp. Ther.
312(2):794-800 (2005)
-
Lauver DA, Lockwood SF and Lucchesi BR. Disodium disuccinate astaxanthin (Cardax) attenuates complement
activation and reduces myocardial injury following ischemia/reperfusion. J Pharm. Exp. Ther. 314(2):686-692 (2005)
- Booth EA, Obeid NR and Lucchesi BR. Activation of estrogen receptor-alpha protects the in vivo rabbit heart from ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 289(5):H2039-47 (2005)
Pig cTnI Publications
-
Wu D, Bassuk J, Arias J, Doods H and Adams JA. Cardiovascular effects of Na+/H+ exchanger inhibition with BIIB513 following
hypovolemic circulatory shock. Shock 23(3):269-274 (2005)
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