Cardiac Biomarker Kits

Life Diagnostics manufactures ELISA kits for cardiac troponin-I, myoglobin, fatty acid binding protein, and myosin light chain-1. Kits are available for species including mouse, rat, rabbit, dog, pig, and monkey. Our kits have been used extensively since 2003 and are widely referenced in the scientific literature. In November 2011 we launched a series of ultra-sensitive cardiac troponin-I kits that can be used for measurement of this important biomarker in both serum and plasma. The Ultra-Sensitive kits are approximately four-fold more sensitive than our High-Sensitivity kits.

IMPORTANT: Serum or plasma that is to be used for measurement of cardiac biomarkers must not be collected by cardiac puncture or any other method that causes damage to the heart. Cardiac puncture causes release of very high levels of cardiac biomarkers into the blood thereby invalidating study results.

Literature references detailing the use of our cardiac biomarker ELISA kits are listed at the bottom of this page

Orders may be placed by phone, fax, email, or via our online shop.

Discounts: 2-3 kits, 10%; >3 kits, 15%

Cardiac Troponin-I ELISA Kits

ELISA Kit

Range (ng/ml) Kit InstructionsCatalog #Price (96-well kit)

Mouse Cardiac Tn-I (for serum)

0.156 – 10Insert2010-1-HS$450

Mouse Cardiac Tn-I (for plasma)

0.156 – 10Insert2010-1-HSP$450

Mouse Cardiac Tn-I, Ultra Sensitive (for serum or plasma)1

0.039 – 2.5 Insert 2010-1-US$500

Rat Cardiac Tn-I (for serum)

0.156 – 10Insert2010-2-HS$450

Rat Cardiac Tn-I (for plasma)

0.156 – 10Insert2010-2-HSP$450

Rat Cardiac Tn-I, Ultra Sensitive (for serum or plasma)1

0.039 – 2.5 Insert 2010-2-US$500 

Dog Cardiac Tn-I (for serum)

0.156 – 10Insert2010-3-HS$450

Dog Cardiac Tn-I, Ultra Sensitive (for serum or plasma)1

 0.039 – 2.5 Insert 2010-3-US$500

Pig Cardiac Tn-I (for serum)

0.039 – 2.5Insert2010-4-HS$450

Pig Cardiac Tn-I (for plasma)

0.039 – 2.5Insert2010-4-HSP$450

Pig Cardiac Tn-I, Ultra Sensitive (for serum or plasma)1

0.0156 – 1.0 Insert 2010-4-US$500 

Monkey Cardiac Tn-I (for serum)

0.156 – 10Insert2010-5-HS$450

Monkey Cardiac Tn-I, Ultra Sensitive (for serum or plasma)1

 0.039 – 2.5Insert2010-5-US $500 

Rabbit Cardiac Tn-I (for serum)

0.156 – 10Insert2010-6-HS$450

Rabbit Cardiac Tn-I, Ultra Sensitive (for serum or plasma)1

0.0195 – 1.25 Insert 2010-6-US$500 

Guinea Pig Cardiac Tn-I (for serum)

0.2 – 12.5Insert2010-7-HS$450

Bovine Cardiac Tn-I (for serum)

0.156 – 10Insert2010-8-HS$450
1The ultra sensitive versions of our cardiac troponin-I kits can be used with serum or plasma samples. They require a total incubation time of three hours and twenty minutes whereas the high sensitivity (HS and HSP) versions of the kits require a total incubation time of one hour and twenty minutes.
 
Myoglobin ELISA Kits*

ELISA Kit

Range (ng/ml) Kit InstructionsCatalog #Price (96-well kit)
Mouse Myoglobin (for serum, plasma, & urine)3.9 – 250Insert2110-1-N$450

Rat Myoglobin (for serum, plasma, & urine)

1.56 – 100Insert2110-2-N$450

Rabbit Myoglobin (for serum, plasma, & urine)

2 – 125Insert2110-3-N$450

Dog Myoglobin (for serum, plasma, & urine)

3.9 – 250Insert2110-4-N$450

Pig Myoglobin (for serum, plasma, & urine)

0.5 – 3.125Insert2110-5-N$450

Monkey Myoglobin (for serum, plasma, & urine)

2 – 125Insert2110-6-N$450

Guinea Pig Myoglobin (for serum, plasma, & urine)

1.56 – 100Insert2110-7-N$450

 

Fatty Acid Binding Protein ELISA Kits*

ELISA Kit

Range (ng/ml) Kit InstructionsCatalog #Price (96-well kit)

Mouse Cardiac FABP (for serum & urine)

0.31 – 20Insert2310-1-HS$450

Rat Cardiac FABP (for serum & urine)

0.078 – 5Insert2310-2-HS$450

Dog Cardiac FABP (for serum & urine)

0.31 – 20Insert2310-4$450

Pig Cardiac FABP (for serum & urine)

0.78 – 25Insert2310-5$450

Rabbit Cardiac FABP (for serum & urine)

1.56 – 100Insert2310-6$450
Monkey Cardiac FABP (for serum)3.9 – 250Insert2310-7$450
 
Cardiac Myosin Light Chain-1 ELISA Kits

ELISA Kit

Range (ng/ml) Kit InstructionsCatalog #Price (96-well kit)
Rat & Mouse Cardiac MLC-1 (for serum)0.078 – 2.5Insert2320-2$450
*Please note that use of urine and plasma may require dilution of samples with the kit dilution buffer in order to avoid matrix effects.

The figure below illustrates the utility of our animal troponin-I, myoglobin, and FABP kits. It shows the levels of the respective markers in plasma obtained from a rabbit cardiac ischemia/reperfusion model. Plasma samples were collected before/during ischemia and at various times after reperfusion. Consistent with clinical findings in humans, it can be seen that  (i) the levels of myoglobin and FABP increase prior to those of troponin-I and (ii) troponin-I levels remain elevated longer than do those of myoglobin and FABP.

Mouse cTnI Publications

  1. Haas MS. Alicot EM, Schuerpf F, Chiu I, Li J, Moore FD and Carrol MC. Blockade of self-reactive IgM significantly reduces injury in a murine model of acute myocardial infarction. Cardiovascular Research 87:618-627 (2010)
  2. Cao Z, Hu Y, Wu W, Ha T, Kelley J, Deng C, Chen Q, Li J and Li Y. The TIR/BB-loop mimetic AS-1 protects the myocardium from ischemia/reperfusion injury. Cardiovascular Research 84:442-451 (2009)
  3. Engle SK. et.al. Qualification of cardiac troponin-I concentration in mouse serum using isoproterenol and implementation in pharmacology studies to accelerate drug development. Toxicologic Pathology 37:617-628 (2009)
  4. Qi D, Hu X. Wu X, Merk M, Leng L, Bucala R and Young LH. Cardiac Macrophage migration inhibitory factor inhibits JNK pathway activation and injury during ischemia/reperfusion. J. Clin .Invest. 119:3807-3816 (2009)
  5. Richer MJ, Lavallée DJ, Shanina I, Horwitz MS (2009) Toll-Like Receptor 3 Signaling on Macrophages Is Required for Survival Following Coxsackievirus B4 Infection. PLoS ONE 4(1): e4127. doi:10.1371/journal.pone.0004127
  6. Atkinson C., et.al. Complememnt-dependent inflammation and injury in a murine model of brain dead donor hearts. Circulation Research. 105:1094-1101 (2009)
  7. Buyse MG, et.al. Long-term blinded placebo-controlled study of SNT-MC17/idebenone in the dystrophin deficient mdx mouse: cardiac protection and improved exercise performance. Eurpoean Heart Journal 30:116-124 (2009)
  8. Elrod JW et.al. Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function. PNAS 104:15560-15565 (2007)
  9. Eckle T, et.al. Cardioprotection by Ecto-5′-Nucleotidase (CD73) and A2B Adenosine Receptors. Circulation 115:1581-1590 (2007)
  10. Eckle T, et.al. Systematic evaluation of a novel model for cardiac ischemic preconditioning in mice. Am J Physiol Heart Circ. Physiol. 291:H2533-H2540 (2006)
  11. Hardarson HS, et.al. Toll-like receptor 3 is an essential component of the innate stress response in virus-induced cardiac injury. Am J Physiol Heart Circ. Physiol 292:H251-H258 (2006)
  12. Madonna R, Rinaldi L, Rossi C, Geng Y-J and De Caterina R. Prostacyclin improves transcoronary myocardial delivery of adipose tissue-derived stromal cells. Clinical Research 27:2054-2061 (2006)
  13. Morrison RR, Tan XL, Ledent C, Mustafa SJ and Hifmann PA. Targeted deletion of A2A adenosine receptors attenuates the protective effects of myocardial postconditioning. Am J Physiol Heart Circ Physiol. 293:H2523-H2529 (2007)
  14. Engle, SK et al.  Strain differences in serum cardiac troponin-I concentration and myocardial necrosis after isoproterenol treatment in mice.  Abstract No. 383.  The Toxicologist CD — An official Journal of the Society of Toxicology, Volume 96, Number S-1, March 2007.
  15. Hou G, Dick R, Zeng C, Brewer GJ., Comparison of lowering copper levels with tetrathiomolybdate and zinc on mouse tumor and doxorubicin models. Translational Research 148:309-314 (2006)
  16. Huber SA, Feldman AM and Sartini D., Coxsackievirus B3 induces T regulatory cells, which inhibit cardiomyopathy in tumor necrosis factor-a transgenic mice. Circ Res. 99:1109-1116 (2006)
  17. Mullick A, Leon Z, Min-Oo G, Berghout J, Lo R, Daniels E and Gros P. Cardiac failure in C5-deficient mice after Candida albicans infection. Infection and Immunity. 74(8):4439-4451 (2006)Hou G, Dick R, Abrams GD, Brewer GJ., Tetrathiomolybdate protects against cardiac damage by doxorubicin in mice. J Lab Clin Med.146(5):299-303 (2005Tao L, Liu H-R, Gao F, Qu Y, Christopher TA, Lopez BL and Ma XL. Mechanical traumatic injury without circulatory shock causes cardiomyocyte apoptosis: rol of reactive nitrogen and reactive oxygen species. Am J Heart Circ Physiol.  288:H2811-H2818 (2005)

Rat cTnI Publications

  1. Todorova VK, Kaufman Y, Hennings LJ and Klimberg VS. Glutamine regulation of doxorubicin accumulation in hearts versus tumors in experimental rats. Cancer Chemother Pharmacol 66:315-323 (2010)
  2. Ji X, Takahashi R, Hiura Y, Hirokawa G, Fukushima Y and Iwai N. Plasma miR-208 as a Biomarker of Myocardial Injury. Clin Chem. 55(11):1944-1949 (2009)
  3. Yue T-L, et.al. In vivo activation of peroxisome proliferator-activated redeptor-d protects the heart from ischemia/reperfusion injury in Zucker fatty rats. J. Pharm. Exp. Ther. 325:466-474 (2008)
  4. Zagorski J, Gellar MA, Obraztsova M, Kline JA and Watts JA. Inhibition of CINC-1 decreases right ventricular damage caused by experimental pulmonary embolism in rats. The Journal of Immunology 179:7820-7826 (2007)
  5. Legare JF, Oxner A, Heimrath O, Myers T and Currie RW. Heat Shock treatment results in increased recruitment of labeled PMN following myocardial infarction. Am J Physiol Heart Circ Physiol. 293(5):H3210-5 (2007)
  6. Chopra M and Sharma AC. Distinct cardiodynamic and molecular chracteristics during early and late stages of sepsis-induced myocardial dysfunction. Life Sciences. epub. July 2007
  7. Liu J, Pang Y, Chang T, Bounelis P, Chatham JC, and Marchase RB. Increased hexoseamine biosynthesis and protein O-GlcNAc levels associated with myocardial protection against calcium paradox and ischemia. J Mol. Cell Cardiol. 40 303-312 (2006)
  8. Wang P and Chatham JC. Onset of diabetes in Zucker diabetic fatty (ZDF) rats leads to improved recovery of function after ischemia in the isolated perfused heart. Am J Physiol Endocrinol Metab 286:E725-E736 (2004)

Rabbit cTnI Publications

  1. Booth EA, and Lucchesi BR. Medroxyprogesterone acetate prevents the cardioprotective and anti-inflammatory effects of 17beta-estradiol in an in vivo model of myocardial ischemia and reperfusion. Am J Physiol Heart Circ Physiol. 293(3):H1408-15 (2007)
  2. Lauver DA, Booth EA, White AJ, Poradosu E & Lucchesi BR. Sulodexide attenuates myocardial ischemia/reperfusion injury and the deposition of C-reactive protein in areas of infarction without affecting hemostasis. J Pharm. Exp. Ther. 312(2):794-800 (2005)
  3. Lauver DA, Lockwood SF and Lucchesi BR. Disodium disuccinate astaxanthin (Cardax) attenuates complement activation and reduces myocardial injury following ischemia/reperfusion. J Pharm. Exp. Ther. 314(2):686-692 (2005)
  4. Booth EA, Obeid NR and Lucchesi BR. Activation of estrogen receptor-alpha protects the in vivo rabbit heart from ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 289(5):H2039-47 (2005)

Pig cTnI Publications

  1. Liu J-Q, Lee T-F, Bigam DL and Cheung P-Y. Effects of post-resuscitation treatment with N-acetylcysteine on cardiac recovery in hypoxic newborn piglets. PLoS one 5(12): e15322 (2010)
  2. Osipov RM, et.al. Effect of hydrogen sulfide on myocardial protection in the setting of cardioplegia and cardiopulmonary bypass. Interactive CardioVascular and Thoracic Surgery 10: 506-512 (2010)
  3. Adams JA, et.al. Periodic acceleration (pGz) prior to whole body ischemia reperfusion injury provides early cardioprotective preconditioning. Life Sciences 86: 707-715 (2010)
  4. Wu D, Bassuk J, Arias J, Doods H and Adams JA. Cardiovascular effects of Na+/H+ exchanger inhibition with BIIB513 following hypovolemic circulatory shock. Shock 23(3):269-274 (2005)

Monkey cTnI Publications

  1. Minimo H, et.al. Characteristics of troponins as myocardial damage biomarkers in cynomolgus monkeys. J. Toxicol. Sci. 34(6): 589-601 (2009)