Immunotoxicity ELISA Kits
Anti-KLH, anti-SRBC, and anti-tetanus toxoid IgM and IgG are routinely used as biomarkers of immunotoxicity. Life Diagnostics manufactures ELISA kits for these important biomarkers in mice, rats, chickens, and monkeys. In preclinical studies, animals are immunized with an immunogen such as KLH while being dosed with a drug candidate. Serum is collected at either 7 or ~ 21 days and the levels of anti-KLH IgM and IgG are determined and compared with those in a control group that was not exposed to the drug. A decrease in anti-KLH levels in the treatment group provides evidence of immunosuppression.
Orders may be placed by phone, fax, email, or via our online shop.
Discounts: 2-3 kits, 10%; >3 kits, 15%
| Anti-KLH IgG & IgM ELISA Kits | |||
| ELISA Kit | Kit Instructions | Catalog # | Price (1 x 96-well) |
| Mouse Anti-KLH IgM | Insert | 4000-1 | $450 |
| Mouse Anti-KLH IgG | Insert | 4010-1 | $450 |
| Rat Anti-KLH IgM | Insert | 4000-2 | $450 |
| Rat Anti-KLH IgM with Controls | Insert | 4000-2C | $500 |
| Rat Anti-KLH IgG | Insert | 4010-2 | $450 |
| Rat Anti-KLH IgG with Controls | Insert | 4010-2C | $500 |
| Guinea Pig Anti-KLH IgG | Insert | 4010-3 | $450 |
| Monkey Anti-KLH IgM (ng/ml) | Insert | 4000-4-N | $500 |
| Monkey Anti-KLH IgG1 (ng/ml) | Insert | 4010-4-1 | $500 |
Anti-Sheep Red Blood Cell IgG & IgM ELISA Kits | |||
| ELISA Kit | Kit Instructions | Catalog # | Price (1 x 96-well) |
| Rat Anti-SRBC IgM | Insert | 4200-2 | $450 |
| Rat Anti-SRBC IgG | Insert | 4210-2 | $450 |
| Mouse Anti-SRBC IgM | Insert | 4200-1 | $450 |
| Mouse Anti-SRBC IgG | Insert | 4210-1 | $450 |
| Chicken Anti-SRBC IgM | Insert | 4200-5 | $450 |
| Chicken Anti-SRBC IgG | Insert | 4210-5 | $450 |
Anti-Tetanus Toxoid IgG & IgM ELISA Kits | |||
| ELISA Kit | Kit Instructions | Catalog # | Price (1 x 96-well) |
| Rat Anti-Tetanus Toxoid IgM | Insert | 4300-2 | $450 |
| Rat Anti-Tetanus Toxoid IgG | Insert | 4310-2 | $450 |
| Monkey Anti-Tetanus Toxoid IgM (ng/ml) | Insert | 4300-4-N | $500 |
| Monkey Anti-Tetanus Toxoid IgG1 (ug/ml) | Insert | 4310-4-1 | $500 |
Our rat and monkey anti-KLH IgG ELISA kits have recently been evaluated and gained unqualified approval in a respected independent laboratory (Validation of an ELISA assay for quantification of anti-KLH IgG in rat and monkey sera. CIT, Evreux, France. Armelle N. Guyen, Sandrine Arthaud, Phillipe Ancian and Roy Forster. Toxicology Letters, Volume 180, Supplement 1, 5 October 2008, Page S211).
Publications describing the use of LDI Immunotoxicity kits
- Guyen AN, Arthaud S, Ancian P and Foster R. Validation of an ELISA assay for quantification of anti-KLH IgG in rat and monkey sera. Toxicology Letters (Abstracts) 180S: S211/I11 (2008)
- Betz BC, Jordan-Williams KL, Wang C, Kang SG, Liao J, Logan MR, Kim CH and Taparowsky EJ. Batf coordinates multiple aspects of B and T cell function required for normal antibody responses. J. Exp. Med. 207(5):933-942 (2011) [Mouse anti-SRBC IgG & IgM] http://jem.rupress.org/content/207/5/933.full.pdf+html
- Schaffer BS, Grayson MH and Wortham JM, et al. Immune competency of a hairless mouse strain for improved preclinical studies in genetically engineered mice. Mol. Cancer Ther. 9:2354-2364 (2010) [Mouse anti-KLH IgG & IgM] http://mct.aacrjournals.org/content/9/8/2354.full.pdf+html
- Efanov A, Zanesi N, NazaryanN, Santanam U, Palmarchuck A Croce CM and Pekarsky Y. CD5+CD23+ leukemic cell populations in TCL1 transgenic mice show significantly increased proliferation and Akt phosphorylation. Leukemia 24:970-975 (210) [Mouse anti-SRBC IgM]
- Nain S and Smits JEG. Pathological, immunological and biochemical markers of subchronic arsenic toxicity in rats. Environmental Toxicology. (2010) http://onlinelibrary.wiley.com/doi/10.1002/tox.20635/abstract [Rat anti-KLH IgG & IgM]