PEG Reagents

We manufacture four mouse monoclonal anti-PEG antibodies that recognize the PEG polymer backbone with differing affinities and a PEG ELISA kit can that can be used for detection of PEG chains > 5kDa. We also offer mono-PEGylated BSA of 5 kDa and 20 kDa for use as controls or standards and have additional projects underway aimed at generating monoclonal antibodies against the terminal methoxy group of PEG.

Orders may be placed by phone, fax, email, or via our online shop.

 

Product

Instructions / Info sheet

Catalog #

Price

PEG ELISA Kit (High Sensitivity)
- Competitive ELISA
- PEG-backbone specific
- Uses LDI monoclonal 1D9-6
- ng/ml sensitivity for free PEG and PEGylated proteins
- Rapid assay: 80 min incubation time

 Kit Insert P-0003 $500 / kit

Anti-PEG Monoclonal, Clone 1D9-6
- Useful in indirect ELISA & western blot
- Backbone specific
- Recognizes PEG with mwt of 550 Da or greater in indirect ELISA
- Kd = 2.88 x 10-9 M

Info

1D9-6

$2000 / 1 mg
$250 / 100 ug

Anti-PEG Monoclonal, Clone 3F12-1
- Useful in indirect & sandwich ELISA
- Backbone specific
- Recognizes PEG with mwt of 550 Da or greater in indirect ELISA
- Kd = 1.84 x 10-8 M		Info3F12-1

$1500 / 1 mg
$200 / 100 ug

Anti-PEG Monoclonal, Clone 10B4-2
- Useful in direct, indirect & sandwich ELISA
- Backbone specific
- Recognizes PEG with mwt of 550 Da or greater in indirect ELISA
- Kd = 2.28 x 10-8 M		 Info10B4-2$1500 / 1 mg
$200 / 100 ug
Anti-PEG Monoclonal, Clone 10E3-1-4
- Useful in ELISA & Western Blot
- Backbone specific
- Recognizes PEG with mwt of 550 Da or greater in indirect ELISA
- Kd = 3.7 x 10-8 M		Info10E3-1-4

$1500 / 1 mg
$200 / 100 ug

Anti-PEG Monoclonal Sampler Pack
- 100 ug each of Monoclonals 1D9-6, 3F12-1, 10B4-2 and 10E3-1-4		 PMAB-01

$500

Anti-PEG Rabbit Polyclonal IgG Antibody
- Useful in direct, indirect & sandwich ELISA & Western Blot
- Backbone specific – affinity purified on non-methoxy PEG-agarose
- Recognizes PEG with mwt of 550 Da or greater

Info

PEGPAB-01

$1500 / 1 mg
$200 / 100 ug

Mono-PEGylated BSA (mPEG 20 kDa)InfoPBSA-01

$500 / 100 ug

Mono-PEGylated BSA (mPEG 5 kDa)InfoPBSA-02

$500 / 100 ug

 

 

PEG
EC50 (ng/ml)

BSA-(mPEG 20 kDa)3-7

1.0

BSA-mPEG 20 kDa, Kit Standard

3.6

BSA-mPEG 10 kDa

9.4

BSA-mPEG 5 kDa

111

20 kDa  mPEG

0.7

10 kDa  mPEG

0.6

5 kDa PEG

4.7

 

Table 1. Characterization of different BSA-conjugated and free PEG’s in the high sensitivity PEG ELISA (P-0003). PEGss were characterized by EC50 after fitting competition data to sigmoidal dose-response models. Subscripts indicate the approximate number of PEG chains attached per molecule of BSA. mPEG indicates that the PEG chain had a terminal methoxy group. Please note that the concentrations of PEGylated BSA refer only to the polypeptide content and do not account for mass contributed by PEG.